BioRoot® RCS is a paradigm shift for root canal obturation possible?



The introduction of hydraulic calcium silicate materials for use as root canal sealers was the last development of the original mineral trioxide aggregate (MTA) formulation. The first paper reported the use of MTA as a root canal sealer in conjunction with gutta-percha (1). The use of MTA as a sealer resulted in the formation of mineralized tissues and thus it was the first study to look into the process of biomineralization and tissue reactions to the MTA and its calcium releasing ability (2). The use of MTA as a sealer resulted in higher leakage apically than gutta-percha obturation (3).

The mechanism of action of MTA and its hydration mechanisms were reported later (4-6) and this was followed by the development of commercial root canal sealers. The first ones on the market were developed in 2008 by Egeo and Angelus (7). At the same time, a paper about ProRoot Endo sealer developed by Dentsply was also published (8) but this sealer was not released until recently on the market. The choice of sealers available clinically to date is shown in Table 1. Among these sealers is BioRoot® RCS developed by Septodont. This article discusses the composition and properties of this sealer.

Table Choice of commercial tricalcium silicate-based sealers available clinically




The BioRoot® RCS is the simplest formulation as shown in Table 1. It is water-based and the change from cement to sealer depends on the inclusion of a water-soluble polymer that allows material flow. The first use of a water-soluble polymer added to Portland cement to improve the material properties was published in 2005 (9). The use of a water-soluble polymer to create a root canal sealer was reported in 2009 (10). In this research, the various additions of polymer were investigated and their effect on the resultant material properties and hydration characteristics.

The addition of a water-soluble polymer to MTA did not alter the hydration characteristics of the material and resulted in a material with improved properties suitable for use as endodontic sealer cement (10). Furthermore, the novel sealer based on MTA demonstrated adequate setting time and was dimensionally stable.

It had the potential to be used as root canal sealer cement in clinical practice (11). The BioRoot® RCS is presented in a powder and liquid format as shown in Figure 1. The powder is composed of tricalcium silicate as the active cementitious material and zirconium oxide radiopacifier (12). The liquid is composed of water, calcium chloride, povidone, and a water-soluble polymer. The sealer microstructure and elemental analysis are shown in Figure 2 (A, C) and its hydration over a period of 28 days with the formation of the calcium hydroxide is shown in Figure 3.

The elemental analysis has been corroborated in another recent study (13). When placed in solution, the sealer leaches high levels of calcium ions when compared to other tricalcium silicate-based sealers such as Endo sequence BC sealer and MTA Fillapex (13).

 Fig. 1: BioRoot® RCS presentation by Septodont showing the packaging with the container and scoop for the powder and liquid vials.

BioRoot® RCS

Figure A & B

Figure A Figure B

Figure C & D

Figure C  Figure D


Fig. 2: Surface microstructure of BioRoot® RCS showing the main phases present and elemental analyses shown in vitro (A, C) and in contact with the dentine (B, D) indicating chemical changes including the formation phosphates (Reprinted with permission from Xuereb et al. 2015).

Fig. 2

Fig; 3: Hydration of BioRoot® RCS showing the crystalline phases formed after 1 and 28 days after mixing using X-ray diffractometry

(Reprinted with permission from Xuereb et al. 2015).




Setting time

The final setting time of BioRoot® RCS was shown to be 324 (±1) minutes which was shorter than that for AH Plus (15). MTA Fillapex did not set when used as a comparison to other tricalcium silicate-based root canal sealers (14, 15). The setting time of BioRoot® RCS was reduced drastically on the application of heat used in warm vertical compaction obturation techniques (16). The contact with a wet environment lengthened the setting time considerably (14). In fact, the manufacturer recommends the use of BioRoot® RCS with cold obturation techniques only particularly with gutta-percha in a single cone obturation technique.


BioRoot® RCS was shown to be less soluble than AH Plus and MTA Fillapex immediately after immersion in water but its solubility was higher over time when compared to the resin-based sealers (15). The solubility enhances the biological properties of the sealer. Immersion in phosphate-buffered saline improved the BioRoot® RCS solubility in the long term and a surface precipitate was observed after 14 and 28 days of immersion (15).

Flow and film thickness

BioRoot® RCS exhibits a lower flow and higher film thickness (12) than the limits specified by ISO 6976;2012 (17) recommendations. The ISO recommendations are intended for inert sealers, unlike the BioRoot® RCS. The flow and film thickness is affected by the heat applied during the warm vertical compaction procedures (16). The manufacturer in fact recommends the use of cold obturation techniques.


The radiopacity of BioRoot® RCS was shown to be greater than the lower limit specified by ISO6876;2012 (17) and similar to that of AH Plus and MTA Fillapex (15). The radiopacity was shown to be about 9 mm aluminum thickness which is similar to Endosequence BC sealer and higher than for MTA Fillapex (14).

Calcium ion release

BioRoot® RCS was shown to release high levels of calcium in solution, which is much higher than other similar sealer types. In fact, it releases double the amount leached by Endosequence BC sealer and ten times as much as calcium ions released by MTA Fillapex for the same time periods under the same conditions (14). Biomineralization and the deposition of phosphates over the material when in contact with the dentine has been shown (14) as indicated in Figure 2 (B, D).


Contact of tricalcium silicate-based materials with dentine and tissue fluids has been reported to lead to the deposition of phosphates on the material's surface. This has been extensively described for MTA (18-20). The interaction of dentine and Biodentine® has been also well documented. A chemical bond is achieved through a mineral infiltration zone at the material-to-tooth interface (21).

This property is important for sealers as bonding of the sealer to the root canal dentine will lead to less microleakage. The mineral infiltration zone has been reported for BioRoot® RCS using confocal microscopy (22). The mineral infiltration zone and the sealer tags ensure sealer adaption and bonding to the root canal dentine (Figure 4). The tags and mineral-rich zone were more evident in the coronal portion than in mid- root and apically. This could be caused by limited action of the ethylene diamine tetracetic acid (EDTA) irrigation and removal of the smear layer further down in the root canal (23).

The infiltration of phosphorus into the BioRoot® RCS when this sealer is in contact with the dentine has not been proven. Surface phase analysis using grazing angle X-Ray diffractometry did not find the formation of calcium phosphate in the material in contact with dentine. This was demonstrated using an in vitroin vivo model where a low-pressure column filled with a physiological solution was used to assess material setting and the chemical composition when in use. This testing is more reliable than in vitro testing where large volumes of fluid are used which is not a clinically relevant situation (14). To enhance the bonding of the sealer to the root canal wall, a phosphate-buffered saline root canal dressing has been suggested (23). This would lead to the availability of phosphate ions thus enhancing the bonding at the interface. The deposition of calcium phosphate has been implicated in the increase in push-out bond strength of tricalcium silicate-based root canal sealers (24).

When compared to MTA Fillapex and AH Plus, BioRoot® RCS showed the greatest antimicrobial activity. The root canal sealers exerted a higher antimicrobial activity when EDTA was used as the final irrigant. Unfortunately, the antimicrobial properties of the BioRoot® RCS and other sealers of related chemistry including AH Plus showed a reduction in antimicrobial properties when phosphate-buffered saline was used as the final irrigant during root canal therapy (25).

Fig. 4: Confocal microscopic images of BioRoot® RCS interface with dentine at different


Fig. 4: Confocal microscopic images of BioRoot® RCS interface with dentine at different levels along the root canal showing the mineral infiltration zone and sealer tags in dentinal tubules (Reprinted with permission from Viapiana et al. 2016).



Elutions from BioRoot® RCS and even direct seeding of cells over the materials showed a high degree of cell proliferation. Migration of periodontal ligament stem cells was observed to be higher with BioRoot® RCS and the cells maintained their mesenchymal phenotype (26).

This was corroborated by another study testing the elutions from BioRoot® RCS and other tricalcium silicate-based sealers together with AH Plus. The 1-day material elution showed no cytotoxic effect while 48 and 72-hour extracts exhibited mild cytotoxicity (27). The 1-day elution of BioRoot® RCS was also evaluated in another study and no DNA double-strand breaks were observed when compared with other resin- and silicate-based root canal sealers (28). BioRoot® RCS did not compromise the mineralization potential of pulpal A4 stem cells. It was not as cytotoxic as Pulp Canal Sealer which is a zinc oxide eugenol-based material. It did not recruit the pulpal stem cells toward differentiation but preserved their osteo-odontogenic intrinsic properties (29). BioRoot® RCS also showed less toxic effects on periodontal ligament cells than Pulp Canal Sealer and induced a higher secretion of angiogenic and osteogenic growth factors than Pulp Canal Sealer (30).



Obturation with BioRoot® RCS


BioRoot® RCS was affected by the irrigation protocol used. Using EDTA as the final irrigant led to the reduction of calcium releasing ability by half (31). Furthermore, in contact with dentine, the calcium phosphate phase was not formed when EDTA was used as the final irrigant (31) as shown in Figure 5 which compared the crystalline phases formed after using saline or EDTA as the final irrigating solution prior to obturation with BioRoot® RCS. Irrigation with EDTA showed the highest antimicrobial properties for BioRoot® RCS. The antimicrobial activity of BioRoot® RCS was significantly higher than that of MTA Fillapex and AH Plus. BioRoot® RCS showed the greatest antimicrobial activity and this was enhanced by using EDTA irrigating solution (25). The use of phosphate-rich irrigating solutions is contraindicated with BioRoot® RCS and all tricalcium silicate-based sealers. The application of heat during warm vertical compaction affects the flow and film thickness of the BioRoot® RCS. Thus, this sealer is recommended for use with single cone techniques or laterally condensed gutta-percha (16). The choice of sealer should be considered when selecting the obturation technique.

The manufacturer recommends the use of a single cone obturation technique with BioRoot® RCS since this sealer is antimicrobial thus its presence would potentially eliminate any microorganisms left inside the root canal space and in the dentinal tubules. Its high antimicrobial activity is evident and it is still effective whatever the irrigating regime used (25). The retreatability of BioRoot® RCS sealer used in conjunction with gutta-percha in single cone obturation technique was better compared to AH Plus as fewer sealer remnants and shorter retreatment times were observed (32).


Fig. 5

Fig. 5: X-ray diffraction plot of BioRoot® RCS after contact with dentine irrigated with saline or EDTA showing the depletion of calcium phosphate deposits on the material in contact with the dentine after irrigation with EDTA marked with a black arrow (Reprinted with permission from Harik et al. 2016).



BioRoot® RCS should be used in conjunction with a solid cone in any cold obturation technique. The material solubility enhances the reaction of the material with environmental ionic exchange thus favoring a biological response. The BioRoot® RCS is highly antimicrobial and the use of EDTA enhances its antimicrobial activity. This sealer was not developed to conform to the classical recommendations of a hermetic seal as it aims to create an environment within the root canal that enhances biological activity and maintains antimicrobial activity. Thus a paradigm shift is possible with BioRoot® RCS.



Josette Camilleri

Josette Camilleri
B.Ch.D., M.Phil., Ph.D., FICD, FADM, FIMMM, FHEA (UK)
School of Dentistry,
Institute of Clinical Sciences
College of Medical and Dental Sciences
The University of Birmingham
The U.K.



Professor Josette Camilleri obtained her Bachelor of Dental Surgery and Master of Philosophy in Dental Surgery from the University of Malta. She completed her doctoral degree, supervised by the late Professor Tom Pitt Ford, at Guy’s Hospital, King’s College London.

She has worked at the Department of Civil and Structural Engineering, Faculty for the Built Environment, University of Malta, and at the Department of Restorative Dentistry, Faculty of Dental Surgery, University of Malta. She is currently a senior academic at the School of Dentistry, University of Birmingham, the U.K. Her research interests include endodontic materials such as root-end filling materials and root canal sealers, with a particular interest in mineral trioxide aggregate, Portland cement hydration, and other cementitious materials used as biomaterials and also in the construction industry.

Josette has published over 100 papers in peer-reviewed international journals and her work is cited over 4000 times. She is the Editor of “Mineral trioxide aggregate. From preparation to application” published by Springer in 2014. She is a contributing author to the 7th edition of “Harty’s Endodontics in Clinical Practice” (Editor: BS Chong) and “Glass ionomer types of cement in Dentistry” (Editor: SK Sidhu). She is an international lecturer, a reviewer, and a member of the scientific panel of a number of international journals including the Journal of Endodontics, Scientific Reports, Dental Materials, Clinical Oral Investigation, Journal of Dentistry, Acta Odontologica Scandinavica, and Acta Biomaterialia.





1. Holland R, de Souza V, Nery MJ, Otoboni Filho JA, Bernabé PF, Dezan Júnior E. Reaction of dogs’ teeth to root canal filling with mineral trioxide aggregate or a glass ionomer sealer. J Endod. 1999 Nov;25(11):728-30.

2. Holland R, de Souza V, Nery MJ, Bernabé oF, Filho JA, Junior ED, Murata SS. Calcium salts deposition in rat connective tissue after the implantation of calcium hydroxide-containing sealers. J Endod. 2002 Mar;28(3):173-6.

3. Vizgirda PJ, Liewehr FR, Patton WR, McPherson JC, Buxton TB. A comparison of laterally condensed gutta-percha, thermoplasticized gutta-percha, and mineral trioxide aggregate as root canal filling materials. J Endod. 2004 Feb;30(2):103-6.

4. Camilleri J, Montesin FE, Brady K, Sweeney R, Curtis RV, Ford TR. The constitution of mineral trioxide aggregate. Dent Mater. 2005 Apr;21(4):297-303.

5. Camilleri J. Hydration mechanisms of mineral trioxide aggregate. Int Endod J. 2007 Jun;40(6): 462-70.

6. Camilleri J. Characterization of hydration products of mineral trioxide aggregate. Int Endod J. 2008 May;41(5):408-17.

7. Monteiro Bramante C, Demarchi AC, de Moraes IG, Bernadineli N, Garcia RB, Spångberg LS, Duarte MA. Presence of arsenic in different types of MTA and white and gray Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008 Dec;106(6):909-13.

8. Weller RN, Tay KC, Garrett LV, Mai S, Primus CM, Gutmann JL, Pashley DH, Tay FR. Microscopic appearance and apical seal of root canals filled with gutta-percha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid. Int Endod J. 2008 Nov;41(11): 977-86.

9. Camilleri J, Montesin FE, Di Silvio L, Pitt Ford TR. The chemical constitution and biocompatibility of accelerated Portland cement for endodontic use. Int Endod J. 2005 Nov; 38(11):834-42.

10. Camilleri J. Evaluation of selected properties of mineral trioxide aggregate sealer cement. J Endod. 2009 Oct;35(10):1412-7.

11. Camilleri J, Mallia B. Evaluation of the dimensional changes of mineral trioxide aggregate sealer. Int Endod J. 2011 May;44(5):416-24.

12. Khalil I, Naaman A, Camilleri J. Properties of Tricalcium Silicate Sealers. J Endod. 2016 Oct; 42(10):1529-35.

13. Reszka P, Nowicka A, Lipski M, Dura W, Droździk A, Woźniak K. A Comparative Chemical Study of Calcium Silicate-Containing and Epoxy Resin-Based Root Canal Sealers. Biomed Res Int. 2016; 2016:9808432.

14. Xuereb M, Vella P, Damidot D, Sammut CV, Camilleri J. In situ assessment of the setting of tricalcium silicate-based sealers using a dentin pressure model. J Endod. 2015 Jan;41(1): 111-24.

15. Prüllage RK, Urban K, Schäfer E, Dammaschke T. Material Properties of a Tricalcium Silicate- containing, a Mineral Trioxide Aggregate-containing, and an Epoxy Resin-based Root Canal Sealer. J Endod. 2016 Dec;42(12):1784-1788.

16. Camilleri J. Sealers and warm gutta-percha obturation techniques. J Endod. 2015 Jan;41(1): 72-8.

17. International Standard Organisation (2012). ISO 6876; Dentistry — Root canal sealing materials.

18. Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod. 2005 Feb;31(2):97-100.

19. Tay FR, Pashley DH, Rueggeberg FA, Loushine RJ, Weller RN. Calcium phosphate phase transformation is produced by the interaction of the portland cement component of white mineral trioxide aggregate with a phosphate-containing fluid. J Endod. 2007 Nov;33(11): 1347-51.

20. Reyes-Carmona JF, Felippe MS, Felippe WT. Biomineralization ability and interaction of mineral trioxide aggregate and white portland cement with dentin in a phosphate-containing fluid. J Endod. 2009 May;35(5):731-6.

21. Atmeh AR, Chong EZ, Richard G, Festy F, Watson TF. Dentin-cement interfacial interaction: calcium silicates and polyalkenoates. J Dent Res. 2012 May;91(5):454-9.

22. Viapiana R, Moinzadeh AT, Camilleri L, Wesselink PR, Tanomaru Filho M, Camilleri J. Porosity and sealing ability of root fillings with gutta-percha and BioRoot® RCS or AH Plus sealers. Evaluation by three ex vivo methods. Int Endod J. 2016 Aug;49(8):774-82.

23. Reyes-Carmona JF, Felippe MS, Felippe WT. A phosphate-buffered saline intracanal dressing improves the biomineralization ability of mineral trioxide aggregate apical plugs. J Endod. 2010 Oct;36(10):1648-52.

24. Reyes-Carmona JF, Felippe MS, Felippe WT. The biomineralization ability of mineral trioxide aggregate and Portland cement on dentin enhances the push-out strength. J Endod. 2010 Feb;36(2):286-91.

25. Arias-Moliz MT, Camilleri J. The effect of the final irrigant on the antimicrobial activity of root canal sealers. J Dent. 2016 Sep;52:30-6.

26. Collado-González M, García-Bernal D, Oñate-Sánchez RE, Ortolani-Seltenerich PS (3), Lozano A, Forner L, Elena C, Rodríguez-Lozano FJ. Biocompatibility of three new calcium silicate- based endodontic sealers on human periodontal ligament stem cells. Int Endod J. 2016 Sep 26. doi: 10.1111/iej.12703. [Epub ahead of print]

27. Poggio C, Riva P, Chiesa M, Colombo M, Pietrocola G. Comparative cytotoxicity evaluation of eight root canal sealers. J Clin Exp Dent. 2017 Apr 1;9(4):e574-e578.

28. Eldeniz AU, Shehata M, Högg C, Reichl FX. DNA double-strand breaks caused by new and contemporary endodontic sealers. Int Endod J. 2016 Dec;49(12):1141-1151.

29. Dimitrova-Nakov S, Uzunoglu E, Ardila-Osorio H, Baudry A, Richard G, Kellermann O, Goldberg M. In vitro bioactivity of Bioroot® RCS, via A4 mouse pulpal stem cells.Dent Mater. 2015 Nov;31(11):1290-7.

30. Camps J, Jeanneau C, El Ayachi I, Laurent P, About I. Bioactivity of a Calcium silicate-based Endodontic Cement (BioRoot® RCS): Interactions with Human Periodontal Ligament Cells In Vitro. J Endod. 2015 Sep;41(9):1469-73.

31. Harik R, Salameh Z, Habchi R, Camilleri J. The effect of irrigation with EDTA on calcium-based root canal sealers: a SEM-EDS and XRD study. Journal of the Lebanese Dental Association 2016; 49:12-23.

32. Donnermeyer D, Bunne C, Schäfer E, Dammaschke T. Retreatability of three calcium silicate-containing sealers and one epoxy resin-based root canal sealer with four different root canal instruments. Clin Oral Investig. 2017 Jun 22. DOI: 10.1007/ s00784-017-2156-5. [Epub ahead of print].